Genetic Diversity of Turkish Lathyrus L. Landraces Using ISSR Markers

Abstract

Lokalne populacije Lathyrus sativus L. (ukupno 51) i jedna L. clymenum L. sakupljene u Turskoj, kao i jedan kultivar L. sativus, Gürbüz, ocenjene su ISSR markerima. Korišćena su tri ISSR prajmera za ocenu 45 DNK fragmenata, kod kojih su 44 trake bile polimorfne. Frekvencija ocenjivanih traka bila je u opsegu od 0.009 do 0.888, u proseku 0.363. Genotipovi MLT04 i NEV02 ispoljili su visoku sličnost. Najudaljeniji par bio je Gürbüz i MLT02, sa 0.244. Najbliži genotip Gürbüz-u bio je CNR03 sa 0.577. Genetička distance izmedu L. clymenum i L. sativus bila je od 0.353 (uzorak NEV01) do 0.637 (uzorak DEN04), a geneticka distance sa kultivarom Gürbüz bila je 0.375. Procenom genetickih relacija izmedu Lathyrus genotipova dobijene su dve grupe: jedna predstavlja samo varijetet Gürbüz, dok druga obuhvata 52 Lathyrus lokalne populacije. L. sativus i L. clymenum su se jasno odvojile u drugu grupu.; Fifty-one Lathyrus sativus L. landraces and one L. clymenum L. landrace collected from Turkey and one L. sativus cultivar, Gürbüz, were evaluated with ISSR markers in this study to molecular characterization. Three ISSR primers were used and 45 DNA fragment were evaluated, of which 44 bands were polymorphic. The frequencies of scored bands ranged from 0.009 to 0.888 and averaged 0.363. The genotypes MLT04 and NEV02 had high similarity with 0.825 according to pairwise grouping. The furthest pairwise group was Gürbüz and MLT02 with 0.244. The nearest genotype to Gürbüz was CNR03 with 0.577. The pairwise genetic distance between L. clymenum and L. sativus ranged from 0.353 (accession NEV01) to 0.637 (accession DEN04) and pairwise genetic distance to the cultivar Gürbüz was 0.375. Assessment of genetic relationships among Lathyrus genotypes made two main groups. One of them covered only Gürbüz variety and the other covered 52 Lathyrus landrace. L. sativus and L. clymenum separated prominently in the second group. © 2018 Serbian Genetics Society.; © 2018 Serbian Genetics Society.