Toxic Effects of Immunofluorescent Polystyrene Nanoplastics on Rat Testicular Tissue

Abstract

Background and Aim Nanoplastics (NPs), formed by the degradation of plastic materials, are ubiquitous substances with potential negative effects on various ecosystems and organisms. This article aims to investigate toxicological and biochemical NP accumulation in testicular tissue of rats and explore its potential effects on male reproductive health. Methods Our study was conducted on the control (sham) group and two experimental groups receiving 25 mg/kg NPs (LNp) and 50 mg/kg NPs (HNp). Five male rats in each group were administered fluorescent NPs via gavage for one month. At the end of the experiment, testicular tissues were removed, and NPs accumulation, oxidative stress and testicular inflammation were investigated histopathologically and biochemically. Results NPs accumulation was detected in the testicular tissue of LNp and HNp groups. Immature sperm cells and vacuolization were observed, suggesting that spermatogenesis was adversely affected. Oxidative stress was found to be increased in testicular tissue. Toll-like receptor 7 (TLR7) gene expression was evaluated, and inflammation was found to be statistically significant (p < 0.05). Conclusion In conclusion, continuous intake of nanoplastics can lead to their accumulation in testicular tissue, impairing testicular function and potentially causing infertility. Given their increasing presence in the environment, nanoplastics pose significant risks to male reproductive health, necessitating further research and regulatory action to minimize exposure.