Adolescent Intermittent Ethanol Use in Male Rats Do Not Change Cerebellar Cell Numbers but Initiate Astroglial Reaction

Abstract

Alcohol consumption during adolescence causes negative structural changes in the cerebellum and can lead to cognitive and motor skill disorders. Unfortunately, the age at which individuals begin drinking alcohol has decreased in recent years, which has drawn attention to the effects of alcohol on neurological changes during preadolescence. In this study, we investigated the effects of adolescent intermittent ethanol (AIE) exposure on the cellular composition of the cerebellum in male rats, particularly when alcohol consumption begins early. The male rats received eight doses of intermittent intraperitoneal injection of 25% (v/v) ethanol (3 g/kg) or saline from postnatal days (PND) 25 to PND 38. In rats, 28-42 days old corresponds to 10-18 years old in humans. Two hours after the last injection, the cells, neurons, and non-neuronal cells in the cerebellum were immunocytochemically labeled and the total numbers of related cells were calculated using the Isotropic Fractionator method. We found that AIE exposure does not change the cell numbers of the cerebellum in the short term, but it does activate astrocytes in the white matter of the cerebellum. These findings suggest that alcohol use during adolescence impairs the innate immune system and negatively affects brain plasticity. We researched the harms of adolescent alcohol use on the cerebellum cellularity of male rats with the Isotropic Fractionator's new counting method. Adolescent intermittent ethanol exposure did not change total cell, neuron, and non-neuronal numbers, but immunohistochemical labeling with GFAP showed that alcohol use in teenagers breaks down the immune of the intact cerebellum. image