Investigation of Oxidative Stress Parameters in Cattle Infected With Mycobacterium Avium Subsp. Paratuberculosis

Abstract

Paratuberculosis is a zoonotic disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) in cattle. MAP may cause the formation of reactive oxygen species (ROS) by increasing the release of proinflammatory cytokines in the host. Due to the increase in ROS, the oxidant-antioxidant balance may be disrupted and oxidative stress may occur. The aim of the study was to determine the oxidative stress parameters in cattle infected with paratuberculosis. For this purpose, 15 cattle sera that were positive for paratuberculosis and 15 clinically healthy 30 cattle sera were used as the control group. In the samples taken, oxidative stress parameters such as total antioxidant capacity (TAS), total oxidant capacity (TOS), oxidative stress index (OSI), native thiol, total thiol and disulphide levels were evaluated. According to our study results, TOS (21.911±11.80), OSI (37.99±21.40), total thiol (1836.671±877.06) and disulphide (715.420±395.32) values in the paratuberculosis group were significantly higher than TOS (8.538±5.18), OSI (10.24±7.23), total thiol (823.809±289.86) and disulphide (197.936±131.70) values in the control group (P<.001). The TAS (0.588±0.14) value in the paratuberculosis group was significantly lower than the TAS (0.952±0.26) value in the control group (P<.001). No significant difference was found between the two groups in terms of native thiol levels (P>.05). As a result, it was determined that the oxidant-antioxidant balance was disrupted and oxidative stress occurred in MAP infected cattle. Therefore, it was concluded that oxidative stress parameters can be used as biomarkers in the diagnosis and treatment of the disease. © 2024, Ataturk Universitesi. All rights reserved.